Journal: Yonago Acta Medica

Article Title: Cisplatin Induces Cell Death in Rat Adult Kidney Stem/ Progenitor Cell-Derived Kidney Organoids

doi: 10.33160/yam.2023.02.018

Figure Lengend Snippet: Cisplatin-induced cell death in NLS. A) Fluorescence microscopy image depicting the PI uptake (enlarged view of the tubular structure). Bar = 300 µm. B) Fluorescence microscopy image depicting the PI uptake (overview of NLS). Bar = 1 mm. C) PI-positive areas in the NLS after cisplatin exposure (n = 4 under each concentration). **P < 0.01; Student’s t-test.

Article Snippet: Drug treatment and toxicity assay For toxicity assays, organoids cultured in the “half-Matrigel” on day 14 were placed in the differentiation medium supplemented with 0, 10, or 20 μM cisplatin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) up to day 20.

Techniques: Fluorescence, Microscopy, Concentration Assay

Journal: Yonago Acta Medica

Article Title: Cisplatin Induces Cell Death in Rat Adult Kidney Stem/ Progenitor Cell-Derived Kidney Organoids

doi: 10.33160/yam.2023.02.018

Figure Lengend Snippet: Cisplatin-induced inhibition of NLS growth. A) Growth rate from days 14–20 under cisplatin-free conditions (n = 4 for each condition). B) Cisplatin-induced inhibition of NLS growth relative to that without cisplatin treatment (n = 4 for each condition). **P < 0.01; Student’s t-test.

Article Snippet: Drug treatment and toxicity assay For toxicity assays, organoids cultured in the “half-Matrigel” on day 14 were placed in the differentiation medium supplemented with 0, 10, or 20 μM cisplatin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) up to day 20.

Techniques: Inhibition

Journal: BMC Immunology

Article Title: Inhibitory effect of IL-17 on neural stem cell proliferation and neural cell differentiation

doi: 10.1186/1471-2172-14-20

Figure Lengend Snippet: IL-17R expresses in NSCs. Dissociated single cells were incubated in stem cell culture or differentiation medium in 12-well plates for 2 days and processed for immunofluorescence. ( a ) Immunocytochemistry images of neural stem cells cultures labeled for IL-17R (green) and counterstained with the nuclei dye DAPI (blue). ( b ) IL-17R antibody was omitted to serve as control. Magnification ×20 for A, ×20 for B. ( c ) Plot depicts the percentage of IL-17R positive cells. ( d ) RT-PCR for IL-17R expression in NSCs. Primary oligodendrocytes (>93% GalC + ) of wild type B6 mice served as positive control and of IL-17R knockout mice as negative control. Mean percentages were determined from five independent experiments. WT: wild type.

Article Snippet: To induce NSC differentiation, dissociated single cells or small neurospheres were incubated in stem cell differentiation medium (NSC basal medium plus 10% NSC differentiation supplements, Stemcell Technologies) for 7 to 14 days and processed for immunofluorescence.

Techniques: Incubation, Stem Cell Culture, Immunofluorescence, Immunocytochemistry, Labeling, Control, Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control, Knock-Out, Negative Control

Journal: BMC Immunology

Article Title: Inhibitory effect of IL-17 on neural stem cell proliferation and neural cell differentiation

doi: 10.1186/1471-2172-14-20

Figure Lengend Snippet: IL-17 interferes with NSCs differentiation. Dissociated single cells were incubated in stem cell differentiation medium in 12-well plates and treated with IL-17 (25 ng/ml) for 14 days, then were processed for immunofluorescence. IL-17 treatment did not significantly change the number of β-III-tubulin positive cells ( a ), but significantly decreased the numbers of GFAP positive cells ( b ), NG2 positive cells ( c ) and SOX2 positive cells ( d ). Data represent mean ± SE in duplicate samples from three identical experiments. NS, not significant.

Article Snippet: To induce NSC differentiation, dissociated single cells or small neurospheres were incubated in stem cell differentiation medium (NSC basal medium plus 10% NSC differentiation supplements, Stemcell Technologies) for 7 to 14 days and processed for immunofluorescence.

Techniques: Incubation, Cell Differentiation, Immunofluorescence